Induction of Secondary Metabolites by Virginiamycin Inducing Factor, Virginiae Butanolide C

Virginiamycin 생합성 유도인자 Virginiae butanolide C에 의한 2차 대사산물 생산의 유도

  • 김현수 (계명대학교 자연과학대학 미생물학과) ;
  • 강선영 (계명대학교 자연과학대학 미생물학과)
  • Published : 1994.10.01

Abstract

Virginiae butanolide C(VB-C) is one of the butyrolactone autoregulators, which triggers the production of virginiamycin in Streptomyces virginiae. Streptomyces longwoodensis was selected as a test strain to investigate new VB-C functions. When 100 ng/ml of the synthetic VB-C was added into the culture at 5 hour and 0 hour, the initial production time of antibiotics and a dark blue pigment were shortened by 4~6 hours and 2~4 hours, respectively. HPLC analysis revealed the production of several new antibiotics by VB-C addition. In the SDS-PAGE analysis of the total protein from mycelium several new protein bands showed up and the amounts of certain protein bands increased in the presense of VB-C. The existence of specific VB-C binding protein was confirmed from S. longwoodensis in relation to VB-C signal transduction. These results suggest that the VB-C might have an ability to induce the production of secondary metabolites in Streptomy- ces longwoodensis.

Keywords

Streptomyces longwoodensis;virginiae butanolide C(VB-C);VB-C binding protein;autoregulator

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