Induction of Secondary Metabolites by Virginiamycin Inducing Factor, Virginiae Butanolide C

Virginiamycin 생합성 유도인자 Virginiae butanolide C에 의한 2차 대사산물 생산의 유도

  • 김현수 (계명대학교 자연과학대학 미생물학과) ;
  • 강선영 (계명대학교 자연과학대학 미생물학과)
  • Published : 1994.10.01


Virginiae butanolide C(VB-C) is one of the butyrolactone autoregulators, which triggers the production of virginiamycin in Streptomyces virginiae. Streptomyces longwoodensis was selected as a test strain to investigate new VB-C functions. When 100 ng/ml of the synthetic VB-C was added into the culture at 5 hour and 0 hour, the initial production time of antibiotics and a dark blue pigment were shortened by 4~6 hours and 2~4 hours, respectively. HPLC analysis revealed the production of several new antibiotics by VB-C addition. In the SDS-PAGE analysis of the total protein from mycelium several new protein bands showed up and the amounts of certain protein bands increased in the presense of VB-C. The existence of specific VB-C binding protein was confirmed from S. longwoodensis in relation to VB-C signal transduction. These results suggest that the VB-C might have an ability to induce the production of secondary metabolites in Streptomy- ces longwoodensis.


Streptomyces longwoodensis;virginiae butanolide C(VB-C);VB-C binding protein;autoregulator


  1. J. Bacteriol. v.172 The A-factor binding protein of Streptomyces griseus negatively controls streptomycin and sporulation Miyake,K.;T.Kuzuyama;S.Horinouchi;T.Beppu
  2. J. Ferment. Technol. v.49 Physiological studies on staphylomycin production;(Ⅱ) formation of a substance effective in inducing staphylomycin production Yanagimoto,M.;G.Terui
  3. FEMS Microbiol. Lett. v.28 Reconstitution by a butyrolactone autoregulator of the parental protein pattern in an asporogenous mutant of Streptomyces griseus Grafe,U.;Eva Sarfert
  4. 산업미생물학회지 v.20 Streptomyces virginiae가 생산하는 Virginiae Butanolide C(VB-C) 결합 단백질의 결합활성에 미치는 일반적 특성 김현수
  5. Dokady Akad. Nauk. SSSR v.177 A-factor responsible for the biosynthesis of streptomycin by a mutant strain of Actinomycetes streptomycini Khokhlov,A.S.;I.I.Tovarova;L.N.Borisova;S.A.Pliner;L.A.Shevchenko;E.Ya,Konitskaya;N.S.Ivkina;I.A.Rapoport
  6. J. Antibiot. v.40 The structure of inducing factors for viginiamycin in Streptomyces virginiae Yamada,Y.;K.Sugamura;K.Kondo;M.Yanagimoto;H.Okada
  7. J. Antibiot. v.42 Distribution of virginiae butanolides in antibiotic-producing Actinomycetes, and identification of the inducing factor from Streptomyces antibioticus as virginiae butanolide A Ohashi,H.;Y.H.Zheng;T.Nihira;Y.Yamada
  8. 한국미생물학회지 v.20 Virginiae butanolide C 결합 단백질의 신호 전달기구에 대한 연구 김현수
  9. J. Ferment. Bioeng. v.74 Isolation and structure of a new butyrolactone autoregulator from Streptomyces sp. FRI-5 Ll,W.;T.Nihira;S.Sakuda;T.Nishida;Y.Yamada
  10. J. Antibiot. v.42 Identification of binding protein of virginiae butanolide C, an autoregulator in virginiamycin production, from Streptomyces virginiae Kim,H.S.;T.Nihira;H.Tada;M.Yanagimoto;Y.Yamada
  11. J. Antibiot. v.43 Purification and characterization of virginiae butanolide C-binding protein, a possible pleiotropic signal-transducer in Streptomyces virginiae Kim,H.S.;H.Tada;T.Nihira;Y.Yamada
  12. J. Antibiot. v.253 Lysocellin, a new polyether antibiotic I. isolation, purification, physicochemical and biological properties Ebata,E.;H.Kasahara;K.Secine;Y.Inoue
  13. J. Biol. Chem. v.267 Purification and molecular cloning of a butyrolactone autoregulator receptor from Streptomyces virginiae Okamoto,S.;T.Nihira;H.Kataoka;A.Suzuki;Y.Yamada
  14. J. Bacterol. v.155 Cloning of a pleiotropic gene that positively controls biosynthesis of A-factor, actinorhodin and prodigiosin in Streptomyces coelicolor A3(2) and Sterptomyces lividans Horinouch,S.;O.Hara;T.Beppu
  15. J. Antibiot. v.41 Structure activity relationships of virginiae butanolide C. an inducer of virginiamycin production in Streptomyces virginiae Nihira,T.;T.Shimizu;H.S.Kim;Y.Yamada
  16. 계명대 기초과학 연구소 연구논집 v.12 VB-C에 의한 항생물질 유도방선균의 검색 김현수;강선영
  17. J. Antibiot. v.35 A new inducer of anthracycline biosynthesis from Streptomyces viridochromogenes Grafe,U.;W.shade;I.Eritt;W.F.Fleck;L.Radics
  18. J. Ferment. Bioeng. v.73 IM-2. a butyrolactone autoregulator, induces production of several nucleoside antibiotics in Streptomyces sp. FRI-5 Hashimoto,K.;T.Nihira;S.Sakuda;Y.Yamada
  19. J. Bacteriol. v.158 Unstable genetic determinant of A-factor biosynthesis in streptomycin-producing organism: cloning and characterization Horinouch,S.;S.Y.Kumada;T.Beppu