Molecular breeding of herbicide resistant transgenic plants with bromoxynil specific nitrilase gene

Bromoxynil 특이성 nitrilase 유전자를 이용한 제초제 저항성 형질 전환 식물의 분자육종

  • Min, Bok-Kee (Department of Agricultural Chemistry, College of Natural Resources, Korea University) ;
  • Park, Eun-Sung (Department of Agricultural Chemistry, College of Natural Resources, Korea University) ;
  • Park, Yearn-Hung (Department of Agricultural Chemistry, College of Natural Resources, Korea University) ;
  • Song, Jae-Young (Department of Agricultural Chemistry, College of Natural Resources, Korea University) ;
  • Lee, Se-Yong (Department of Agricultural Chemistry, College of Natural Resources, Korea University)
  • Published : 1994.08.31

Abstract

Bromoxynil is an antidicot herbicide widely used on cereal crops and has a short half life in the soil. A bxn gene, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was inserted in plant binary vector pGA482, and then introduced into tobacco and lettuce plants via Agrobacterium mediated leaf-disc transformation method. Transgenic plants with the bxn gene were selected by kanamycin and regenerated to whole plants. The regenerated transgenic plants were determined level of expression of bxn gene by Northern blot analysis. Leaf-disc analysis and pot-assay confirmed that the transgenic tobacco and lettuce plants were resistant to high doses of bromoxynil.