Inhibition of Radiostrontium Contamination to Fetus by Water Soluble Chitosan in Pregnant Mice

임신마우스에서 수용성카이토산에 의한 태아의 방사성스트론튬 오염 억제

  • Kim, Kwang-Yoon (Department of Nuclear Medicine, Chonnam University Hospital Kwangju) ;
  • Bom, Hee-Seoung (Department of Nuclear Medicine, Chonnam University Hospital Kwangju) ;
  • Yang, Kwang-Hee (Department of Nuclear Medicine, Chonnam University Hospital Kwangju) ;
  • Choi, Keun-Hee (Department of Nuclear Medicine, Chonnam University Hospital Kwangju) ;
  • Chae, Ki-Moon (Department of Nuclear Medicine, Chonnam University Hospital Kwangju) ;
  • Kim, Ji-Yeul (Department of Nuclear Medicine, Chonnam University Hospital Kwangju)
  • Published : 1994.12.30

Abstract

Radiostrontium passes the placental barrier in pregnant rodents very well. Chitosan, a natural nontoxic chelator, was reported to reduce whole body retention of radiostrontium in mice. The aim of the present study was to evaluate water soluble chitosan as a blocking agent of transplacental transfer of radiostrontium in pregnant mice. Twenty pregnant mice were divided into four groups: control and three groups of chitosan treatment (groups 1 to 3). Sr-85(15KBq in 0.2ml saline) was subcutaneously injected into pregnant mice at the 17th day of pregnancy. In control mice, 0.2ml saline was given 5 hours after the injection of Sr-85. In group 1, 1% water soluble chitosan was given subcutanously for two days, twice daily after the injection of Sr-85. In group 2, 10% water soluble chitosan was given orally for 15 days before conception. In group 3, 0.3% water soluble chitosan was injected intravenously for 15 days, once daily before conception. Gamma counting of newborns were done at days 0, 2 and 7 after their births. Whole body retention of Sr-85 in newborns of control mice at days 0, 2, 7 were $3.1{\pm}0.3%,\;2.9{\pm}0.3%,\;2.8{\pm}0.3%$ respectively. In experimental groups, whole body retention of Sr-85 was significantly lower than that of control (p<0.01) and no statistical difference was noted between them. In group 1, the values were $2.1{\pm}0.3%,\;1.4{\pm}0.1%,\;1.4{\pm}0.1%$, respectively. In group 3, they were $2.1{\pm}0.2%,\;1.7{\pm}0.2%,\;1.6{\pm}0.2%$, respectively. In conclusion, the water soluble chitosan reduced transplacental contamination of radiostrontium in pregnant mice.