Genetic Stock Identification of Spotted Flounder, Verasper variegatus from Yeocheun, Korea

범가자미에 대한 유전학적 동정

  • KIM Kyung Kil (National Fisheries Research and Development Agency) ;
  • KIM Yoon (National Fisheries Research and Development Agency) ;
  • NAM Yoon Kwan (Department of Aquaculture, National Fisheries University of Pusan) ;
  • KIM Dong Soo (Department of Aquaculture, National Fisheries University of Pusan)
  • 김경길 (국립수산진흥원 어류양식과) ;
  • 김윤 (국립수산진흥원 어류양식과) ;
  • 남윤권 (부산수산대학교 양식학과) ;
  • 김동수 (부산수산대학교 양식학과)
  • Published : 1993.08.01

Abstract

Cell size, DNA content, chromosome and PCR-based mitochondrial 12S rRNA gene analyses were conducted to obtain basic informations for genetic stock identification of spotted flounder (Verasper variegatus) from Yeocheun, Korea. The mean erythrocytic and nuclear volumes of spotted flounder were $211.10{\mu}m^3$ and $23.03{\mu}m^3$, respectively. The haploid DNA content of this species was 0.79 pg/cell which correspond to $46.5\%$ of carp and to $22.6\%$ of mammals. Spotted flounder had the 2n = 46 acrocentric chromosomes but no heteromorphic sex chromosomes was found. Mitochondrial DNA gene for 12S ribosomal RNA was amplified by polymerase chain reaction (PCR) and the PCR products were subjected to digestion with 15 restriction endonucleases. Restriction enzyme analyses revealed that Ava I, Mae II, Sma I and Xba I had one restriction site in the mitochondrial 12S rRNA gene segment of spotted flounder, while Mae I had two. Segments of 12S rRNA gene from mitochondria in spotted flounder were sequenced and compared with channel catfish and human as controls. The 12S rRNA gene of this species was more similar to that of channel catfish than to human's.

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