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STABLE TRANSFORMATION OF CULTURED CHICKEN CELLS

  • Han, J.Y. ;
  • Shin, Y.S. ;
  • Shoffner, R.N. ;
  • Guise, K.S.
  • Received : 1993.05.31
  • Accepted : 1993.08.13
  • Published : 1993.12.01

Abstract

A plasmid vector, $RSVLTR/{\beta}G2$, containing lacZ gene under the control of the RSVLTR promoter were transfected into chicken embryo fibroblasts by three different transfection methods. Calcium phosphate, lipsome and DEAE-dextran techniques were applied for transfection of chicken cells. A histochemical assay with X-gal was used as a simple method for screening transfected cells. Plasmid $RSVLTR/{\beta}G2$ was expressed proficiently in the chicken embryo fibroblast. Calcium phosphate-DNA precipitate transfection resulted in the highest efficiency for transient expression of $RSVLTR/{\beta}G2$. Transfected cells formed colonies on the 9th day of incubation indicating stable transformation of the inserted plasmid.

Keywords

Plasmid Vector;Transfection;Calcium Phosphate;Liposome;DEAE-Dextran;Chicken Fibroblast