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SEQUENCE ANALYSIS AND COMPARISON OF BOVINE αS1-CASEIN GENOMIC DNA

  • Lin, C.S. (Institute of Animal Science, National Chung Hsing University) ;
  • Huang, M.C. (Institute of Animal Science, National Chung Hsing University) ;
  • Choo, K.B. (Recombinant DNA Laboratory, Department of Medical Research, Veterans General Hospital) ;
  • Tseng, Y.H. (Institute of Molecular Biology, National Chung Hsing University)
  • Received : 1992.11.30
  • Accepted : 1993.07.21
  • Published : 1993.12.01

Abstract

A phage clone containing the partial ${\alpha}_{S1}$-casein gene was isolated from a bovine genomic library by using mixed probes of ovine ${\alpha}_{S1}$-, ${\beta}$- and ${\kappa}$-casein cDNAs. Restriction enzyme mapping analysis for 14.6 kb revealed that the map was in conflict with the report of Meade et al. (1990), especially in the 3'-end fragment. Sequence analysis of 12.6 kb revealed a high AT/GC ratio (1.64); we have identified eight exon sequences according to the bovine ${\alpha}_{S1}$-casein cDNA sequence. The same exon/intron splice junction sequence was observed between these exons. We suggest that the bovine ${\alpha}_{S1}$-casein gene night contain a minimum of 18 exons and the full length is approximately 18-19 kb.

Keywords

Bovine ${\alpha}_{S1}$-Casein Gene;Genomic DNA;Restriction Map;Exon/Intron

Acknowledgement

Supported by : National Science Council of ROC