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EFFECT OF GONADOTROPHINS ON FOLLICULAR STEROID HORMONE PRODUCTION IN HYPOPHYSECTOMISED HENS (Gallus domesticus)

  • Li, Z.D. (Laboratory or Animal Biochemistry, College or Biological Science, Beijing Agricultural University) ;
  • Koga, O. (Department of Animal Sciences, Kyushu Tokai University) ;
  • Tanaka, K. (Department of Animal Sciences, College of Agriculture Kyushu University) ;
  • Fujihara, N. (Department of Animal Sciences, College of Agriculture Kyushu University)
  • Received : 1993.03.24
  • Accepted : 1993.06.10
  • Published : 1993.09.01

Abstract

We assessed effects of ovine luteinizing hormone (oLH) and follicle-stimulating hormone (oFSH) on the granulose and theca layers from the four largest follicles, $F_1-F_4$ of hens which had been hypophysectomized 12 h before expected ovulation. Ovine LH (0.4 mg), oFSH (0.4 mg) or oLH in combination with oFSH (0.4 mg each) was injected intravenously 6 h after hypophysectomy. Progesterone, testosterone and $estradiol-17{\beta}$ levels of the granulose and theca layers which were removed 6 h after hormone injection, were measured by radioimmunoassay. Progesterone contents of $F_1-F_3$ granulosa layer at 12 h after hypophysectomy were much lower than those of control hens. This reduced progesterone level was restored partially by the injection of oLH alone for $F_1$, while no follicles responded to oFSH treatment. In contrast, the injection of oLH in combination with oFSH resulted in high progesterone content of the granulose layer from all four follicles. Progesterone content of the theca layer was negligible in all treatments. Simultaneous injection of oLH and oFSH also elevated $estradiol-17{\beta}$ level accumulating in the theca layer from all follicles, of which much higher concentrations of $estradiol-17{\beta}$ were observed when comparison were made to each of their corresponding controls. No appreciable change in testosterone contents of two layers was observed in the present experiments. These results suggest that oFSH augments function of oLH to stimulate the production of progesterone in the granulose layer and $estradiol-17{\beta}$ in the theca layer.

Acknowledgement

Supported by : Ministry of Education, Science and Culture