Studies on the Insecticide Resistance of the German Cockroach(Blattella germanica L.). III. Comparison of Esterase Activity

바퀴(Blattella germanica L.)의 살충제 저항성에 관한 연구. 3. Esterase활성비교

  • 방종렬 (충북대학교 농생물학과) ;
  • 김정화 (충북대학교 농생물학과) ;
  • 이형래 (충북대학교 농생물학과)
  • Published : 1993.09.01

Abstract

The German cockroach(Blattelia germanica) population~ were successIVely selected with ch\orpyrifos and permethrin during the six generations. The resulting resistant $R_{chtorpenfos}$(Rc) and $R_{permethnn}$(Rp) stra.ins were studied to investigate the esterase activity by spectrophotometer, filter parper test, and electrophoresis. Esterase-$\alpha$ activities by filter paper test showed 2.65 and LBZ times higher in the Rc and Rp strains than the susceptible strain, respectively. ln the spectrophoLometer method, the esterase activit18s to $\alpha$-and $\beta$-naphthyl acetate were increased 2.34 and 5.28 times in the Rc than susceptible strain, and 1.48 and 2.92 times in the Rp Limn susceptible stram, respectlvely. Zymogram patterns of eslerase isozyme by agarose gel electrophoresis showed totally five bands. The Rc and Rp strains showed two additive bands as, Est-2 and Est-3, which were not shown in the susceptible strain. but the Rp strain dId not show Est-5 bands which was COlumon in the Rc and susceptible strams.