Terminal Amino Acid Sequences of Alkaline Amylase from Alkalophilic Bacillus sp. MB 809 and Their Homology

호알카리성 Bacillus sp. MB 809의 알카리성 아밀라제의 말단 아미노산 서열과 그 상동성

  • Moo, Bae (Department of Biology Science, Ewha Womans University) ;
  • Kang, Kyung (Research Center for Molecular Microbiology, Seoul National University)
  • Published : 1993.04.01

Abstract

Alkaline .alpha.-amylase expressed in the transformant, Baciollus subtills MB809, containing alkaline amylase gene cloned from alkalophilic Bacillus sp. AL-8, was purified through for step separation processes. The purified alkaline .alpha.-amylase had molecular weight of app[roximately 59, 000 daltons on SDS-PAGE and Sephaex G-100 gel filtration. Amino acid sequence of terminal portion of the enzyme was analyzed with pure amylase eluted form the SDS-PAGE gel. N-terminal amino acid sequence of .alpha.-amylase was determined by the Edman degradation method and resulted in $NH_{2}$-ser-thr-ala-pro-ser-(ile)-lys-ala-gly-thr-(ile)-leu. For C-terminal amino acid sequencing, purified .alpha.-amylase was digested with carboxypuptidase A and B, and reverse-phase HPLC gradient elution system resulted in -thr-trp-pro-lys-COOH.

Keywords

Alkalophilic Bacillus sp. alkaline amylase;amino acid sequence