Characterization of Mitochondrial Plasmids from Pleurotus spp.

Pleurotus속 균주들의 미토콘드리아 플라스미드 특성

  • 김은경 (서울대학교 자연과학대학 미생물학과) ;
  • 구용범 (서울대학교 자연과학대학 미생물학과) ;
  • 차동렬 (농촌진흥청 농업기술연구소 균이과) ;
  • 하영칠 (서울대학교 자연과학대학 미생물학과) ;
  • 노정혜 (서울대학교 분자미생물학연구센터)
  • Published : 1993.04.01

Abstract

Plasmid DNAs were detected from the mitochondrial fraction of four strains of whiterot fungus, Pleurotus ostreatus. The size of the plasmids were 10.2 and 7.2 kb in strain NFFA 2, 10.2 kb in NFFA 4001, 11.2 kb in NFFA 4501, and 10.2 and 11.2 kb in KFCC 11635. The two strains,NFFA 2ml and NFFA 2m2, which are mutant derivatives of NFFA 2, did not contain any plasmids. The cleavage by proteinase K indicated that these plasmids have DNA ends associated with proteins. In digestion with proteinase K all the plasm ids remained resistant to lambda exonuclease which hydrolyzes DNA from 5' ends and were sensitive to exonuclease III which hydrolyzes DNA from 3' ends. This suggests that the plasmids are linear double-stranded DNA and the terminal proteins are covalently linked to 5' ends of plasm ids. In order to find relationship between these plasmids, hybridization of plasm ids by each separate plasmid DNA was done. The result indicated that the plasmids can be classified into at least 3 groups. Plasmids of group I were present in all the P ostreatus. More mitochondrial plasmids were detected in P cornucopiae. P ,florida, P pulmonarius, P sajor-caju, and P spodoleucus. The size of plasmids ranged between 7.2 kb and 14 kb. All the species except P cornucopiae contained plasmids of approximately 10 kb which hybridized with the 10.2 kb plasmid (group I) of P ostreatus NFFA 2.

Keywords

Pleurotus ostreatus;mitochondrial plasmid;linear plasmid;terminally associated protein;P. cornucopiae;P. florida;P. pulmonarius;P. sajor~caju;P. spodoleucus