Expression of Aspergillus awamori Glucoamylase Gene in Asperillus nidulans

Aspergillus nidulans내에서 Aspergillus awamori의 Glucoamylase 유전자 발현

  • 김석준 (서울대학교 생물교육과, 세포분화연구센터) ;
  • 유준희 (서울대학교 생물교육과, 세포분화연구센터) ;
  • 정구홍 (서울대학교 생물교육과, 세포분화연구센터)
  • Published : 1993.04.01

Abstract

The A. nidulans expression vector which contained trpC marker gene from A. nidulans was constructed to produce glucoamy]ase. The recombinant plasmid was introduced into auxotrophic mutant A. nidulans B17. Southern blot analysis of the genomic DNA from transformant showed that pKHG2 DNA had integrated into the A. nidulans chromosomes. Northern analysis of the total RNA from transform ant showed that mRNA of glucoamylase gene was synthesized in induction condition. Specific activity of glucoamylase was increased in transform ants. G]ucoamylase was shown to be active in non-denaturing acrylamide gel.

Keywords

A. nidulans;transformation;glucoamylase gene expression