Cloning and Expression of pcbAB Genes from Pseudomonas sp. DJ-12 in Escherichia coli

Pseudomonas sp. DJ-12 pcbAB 유전자의 Escherichia coli에서의 클로닝 및 발현

  • 한재진 (충북대학교 자연대 미생물학과) ;
  • 성태경 (충북대학교 자연대 미생물학과) ;
  • 김치경 (서울대학교 분자미생물학 연구센터)
  • Published : 1993.04.01

Abstract

The pchAB genes of Pseudomonas sp. DJ-12 produce the enzymes of 4-chlorobipheny] (4CB) dioxygenase and dihydrodiol dehydrogenase which act on the first and second steps in degradation of 4CB and biphenyl. The genes were cloned in E coli XLI-Blue. The pcbAB genes of about 2.2 kb in size were contained in the pCUlO1 hybrid plasmid in the cloned cell of CUIOI. The genes were found to have their own promoter and three restriction sites for HindlII. 2,3-dihydroxybiphenyl was detected by the resting cell assay, as the metabolite transformed from biphenyl by the cloned cell of CUIOI. This means that the pcbAB genes are well expressed in E. coli. But dechlorination was unlikely involved in the pchAB gene expression but was believed to occur by functioning on 4CBA produced after ring-cleavage of 4CB.

Keywords

pchAB;pchABCD;cloning and expression;biodegradation of 4CB/BP