Identification of the IL-1$\beta$ inhibitor in the febrile patient urine by anti-IL-1$\beta$ monoclonal antibody

Anti-IL-1$\beta$ 단일클론 항체를 이용해서 발열환자의 뇨중 IL-1$\beta$ inhibitor의 확인

  • 남경수 (한국과학기술연구워 유전공학연구소 번역화학실) ;
  • 배윤수 (한국과학기술연구워 유전공학연구소 번역화학실) ;
  • 남명수 (한국과학기술연구원 유전공학연구소 세포생물학실) ;
  • 오은숙 (한국과학기술연구워 유전공학연구소 번역화학실) ;
  • 박순희 (한국과학기술연구원 유전공학연구소 세포생물학실) ;
  • 최인성 (한국과학기술연구원 유전공학연구소 세포생물학실) ;
  • 정태화 (한국과학기술연구워 유전공학연구소 번역화학실)
  • Published : 1993.08.01

Abstract

To effectively purify of IL-1 inhibitor from human febrile urine, we have established monoclonal antibody that reacts with human recombinant interleukin l$\beta$(IL-1$\beta$). The antibody, designated ON-1, was highly specific to IL-1$\beta$ and no cross-reaction with other cytokines(IL-l$\alpha$ and IL-4) was observed. As the results of ELISA inhibition assay and Western blotting method, it was further identified that ON-1 had high binding specificity with IL-1$\beta$. IL-1 receptor binding material from febrile patient urine was effectively purified with affinity column chromatography which conjugated with ON-1. This urinary material inhibited the thymocyte proliferation in a dosedependent manner. IL-l$\beta$ induced thymocyte proliferation activity was inhibited to 67.3% at 6 $\mu\textrm{g}$ of the purified urinary material. The result may suggest that this urinary material the purified urinary material. The result may suggest that this urinary material will have antagonic effect on IL-1 action mechanism and act IL-l$\beta$ inhibitor.