Screening of zearalenone-producing strains by ELISA method

ELISA법에 의한 zearalenone 생성균주의 검색

  • Kim, Sung-Young (Department of Food Science and Technology, Gyeongsang National University) ;
  • Chung, Sun-Hee (Department of Food Science and Technology, Gyeongsang National University) ;
  • Chung, Duck-Hwa (Department of Food Science and Technology, Gyeongsang National University)
  • Published : 1993.02.28

Abstract

ELISA method was applied for the screening of zearalenone producing strains. The developed ELISA was as follow: $125\;{\mu}l$ of diluted solution (1 : 500) of antibody was added to each microtiter well and incubated overnight at $40^{\circ}C$. For direct competitive ELISA, samples and zearalenone-peroxidase conjugate were mixed in a 1 : 1 ratio, and a $100\;{\mu}l$ of aliquot was then added to antisera-coated wells. Plates were incubated for 30 minutes at $37^{\circ}C$, and wells washed 6 times, and $100\;{\mu}l$ of ABTS substrates was added. Plates were incubated for antother 15 minutes at $37^{\circ}C$, and $100\;{\mu}l$ of stopping reagent was added to the wells and absorbance was recorded at 410nm on ELISA Reader. Among 19 strains showed zearalenone-producing ability by ELISA, 3 strains (R-5, C-46, S-134) produced more than 50 ng/ml of zearalenone.