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THE EFFECTS OF PROSTAGLANDINS AND INDOMETHACIN ON OVULATION IN THE PERFUSED FOWL OVARY

  • Tanaka, K. (Laboratory of Animal Breeding, Faculty of Agriculture, Kyushu University) ;
  • Higuchi, T. (Faculty of Agriculture, Kyushu University) ;
  • Tawarazumita, M. (Faculty of Agriculture, Kyushu University) ;
  • Hertelendy, F. (Department of Obstetrics and Gynecology, St. Louis University School of Medicine)
  • Received : 1990.11.08
  • Accepted : 1991.11.25
  • Published : 1992.03.01

Abstract

The effects of prostaglandins (PG) and indomethacin, a PG synthesis inhibitor, on ovulation in the perfused fowl ovary were studied. Laying hens were killed by cutting the jugular vein 18-19 h before expected ovulation of the second follicle of a clutch sequence. The cannulated ovary was dissected free and connected with a recycling perfusion apparatus. Agents to be studied for their effects on ovulation were added to the perfusion fluid. $PGF_{2{\alpha}}$ at 0.1 and 1 mg/l induced ovulation, with a success rate of 25% and 30%, respectively. The same doses of $PGE_2$ were effective at 60% and 63%, respectively. Indomethacin partially blocked gonadotrophin-induced ovulation. It is suggested that PGs may play a supportive role in the process of follicular rupture in the domestic fowl.

Keywords

Fowl Ovary;Perfusion;Prostaglandin;Indomethacin

Acknowledgement

Supported by : Ministry of Education, Science and Culture