A Study on the Nucleotide Analysis of 18S rRNA and the Molecular Evolution of the Korean Decapods(II)

한국산 십각류의 18S 리보솜 RNA의 염기분석과 분자진화에 관한 연구(II)

  • Kim, Won (Department of Molecular Biology, College of Natural Sciences, Seoul National University) ;
  • Min, Gi-Sik (Department of Molecular Biology, College of Natural Sciences, Seoul National University) ;
  • Kim, Sang-Hee (Department of Molecular Biology, College of Natural Sciences, Seoul National University)
  • 김원 (서울대학교 자연과학대학 분자생물학과) ;
  • 민기식 (서울대학교 자연과학대학 분자생물학과) ;
  • 김상희 (서울대학교 자연과학대학 분자생물학과)
  • Published : 1992.10.01

Abstract

The primary sequence of the 18S rRNA gene of a crustacean Pugettia quadridens (Decapoda: Pleocyemata: Brachyura) was determined by the PCR cloning and Taq sequencing. The 18S rRNA gene of this species in 1837 bases long, and 46 bases shorter than that of another crustacean decapod Oedignathus inermis. The similarity between two species is 90.8% when the insertion and/or deletion sites were excluded. Within the molecule, the most conservative (identical) region locates at the position of 1137-1206 and it is 70 bases long. The most long consecutive nucleotide differences occur at the position between 46-55 and the second most between 399-407. The sequence variation in the primary structure of 18S rRNA gene are not evenly distributed throughout the molecule.