Effect of Amino Acids and Dissolved Oxygen on Expression of Invertase in Recombinant Saccharomyces cerevisiae

재조합 Saccharomyces cerevisiae의 Invertase 발현에 미치는 아미노산과 용존산소의 영향

  • 신해헌 (연세대학교 식품공학과) ;
  • 조정섭 (연세대학교 식품공학과) ;
  • 변유량 (연세대학교 식품공학과) ;
  • 박혜영 (연세대학교 식품공학과)
  • Published : 1992.06.01

Abstract

In order to improve the productivity of invertase by recombinant Saccharomyces cerevisiae containing SUC2 gene, the effect of amino acids and dissolved oxygen concentration on the gene expression was investigated. Optimal concentrations of leucine and histidine for cell growth and cloned gene expression were 0.03 gig and 0.04 gig, respectively, expressed as the ratio of amino acid/glucose. The lack or excess of leucine and histidine has inhibitory effect on cell growth and invertase expression. In batch culture, the less aeration was, the higher invertase activity was. In continuous culture at a dilution rate of 0.09 h 1 with controlled dissolved oxygen tension, invertase activity increased dramatically at DOT levels below 5% air saturation, and a maximum activity of 215.54 KUlg cell was obtained under unaerated condition.

Keywords

SUC2 gene;Saccharomyces cerevisiae;dissolved oxygen effet;amino acid effect