Production of Molecular Hydrogen by Automatically Controlled Semi-continuous Outdoor Culture Using Immobilized Cells of Rhodopseudornonas sphaeroides

Rhodopseudomonas sphaeroides 고정화균체의 자동조절 옥외 반연속배양에 의한 수소생산

  • Kim, Jihn-Sang (Department of Microbiology, National Fisheries University of Pusan) ;
  • Bae, Moo (Department of Biology, Ewha Womans University)
  • 김진상 (부산수산대학교 미생물학과) ;
  • 배무 (이화여자대학교 생물학과)
  • Published : 1992.06.01


For the photoproduction of molecular hydrogen by photosynthetic bacteria in outdoor conditions, we constructed automatically controlled semi-continuous culture system. When the amount of hydrogen gas produced can be measured by a gas meter with a pulse generator, the same amount of substrate consumed for hydrogen production could be supplied by micro pump related with timers. Using the apparatus, we examined hydrogen production with immobilized cells of Rhodopseudomonas sPhaeroides B6 in outdoor conditions. In spite of severe fluctuation of weather and illumination, the culture was maintained under good control with regard to hydrogen productivity. It was possible to automate the semi-continuous outdoor culture of photosynthetic bacteria for hydrogen production.


Hydrogen production;automatic outdoor culture;immobilized R. sphaeroides cells.