Studies on the Escherichia coli Hemolysin Antigenic Sites and Functional Sites for the Hemolysin Vaccine Development

Hemolysin 백신 개발을 위한 요로계 감염 대장균들의 Hemolysin Antigenic Sites, Functional Sites 상동성 연구

  • 지근억 (한림대학교 식품영양학과) ;
  • 백광현 (한림대학교 식품영양학과)
  • Published : 1992.06.01

Abstract

This work was performed to investigate the possibility of using J96 hemolysin(Hly, Hly A) vaccine against urinary tract infecting Escherichia coli. Based on the known sequence of J96 hemolysin which was originally isolated from a pyelonephritis patient, ten 20-mer oligonucleotide probes were synthesized. Radioactive labelled 8 probes showed positive colony blots against most of the hemolysin producing wild type E. coli, while HA484 and HA661 showed 28.3, 71.7% positive blots, respectively. This result means that hemolysin genes are highly conserved. Also, 12 anti-Hly MABs(monoclonal antibodies) showed more than 90% positive immunoblots against secreted hemolysin from wild type E. coli. Especially, the result that MAB132 neutralized hemolysin from all of the wild type E. coli augments the idea that hemolysin will be effective as a vaccine.

Keywords

Hemolysin vaccine;antigenic;functional;urinary tract infecting E. coli