Characteristics and Action Pattern of Alkaline Protease from Streptomyces gviseus HC-1141

Streptomyces griseus HC-1141이 생성하는 Alkaline Protease의 특성 및 작용양상

  • Choi, Cheong (Department of Food Science & Technology, Yeungnam Univ.) ;
  • Chung, Yung-Gun (Department of Food Science & Technology, Yeungnam Univ.) ;
  • Sung, Sam-Kyung (Department of Food Science & Technology, Yeungnam Univ.) ;
  • Choi, Kwang-Soo (Department of Food Science & Technology, Yeungnam Univ.) ;
  • Lee, Jae-Sung (Department of Food Science & Technology, Yeungnam Univ.) ;
  • Cho, Young-Je (Department of Food Science & Technology, Yeungnam Univ.) ;
  • Chun, Sung-Sook (Department of Food Science & Technology, Yeungnam Univ.)
  • 최청 (영남대학교 식품가공학과) ;
  • 정영건 (영남대학교 식품가공학과) ;
  • 성삼경 (영남대학교 식품가공학과) ;
  • 최광수 (영남대학교 식품가공학과) ;
  • 이재성 (영남대학교 식품가공학과) ;
  • 조영제 (영남대학교 식품가공학과) ;
  • 천성숙 (영남대학교 식품가공학과)
  • Published : 1992.06.01

Abstract

An alkaline protease producing microorganism was isolated from soil and identified as Streptomyces griseus HC-1141. The optimum pH and temperature for the purified enzyme activity were 8.0 and $60^{\circ}C$, respectively. The enzyme was relatively stable in the pH range of 7.0-9.0 and at the temperature below $60^{\circ}C$. The activity of purified enzyme was inhibited by $Hg^{2+}$, $Cu^{2+}$, $Zn^{2+}$, $Ba^{2+}$ and $Fe^{2+}$, whereas activated by $Mn^{2+}$ and $Ca^{2+}$. $\varepsilon$-Amino caproic acid, 2,4-dinitrophenol and iodine did not show inhibitory effect on the activity of alkaline protease, but p-chloromercuribenzoic acid, ethylendiaminetetraacetic acid showed inhibitory effect on the enzyme activity. These result suggested that the protease was metalloenzyme, and require a reactive SH group for the activity. The reaction of this enzyme follows typical Michaelis-Menten kinetics with the $K_m$ value of $2.229{\times}10^{-4}$M and the $V_{max}$ of $46.08 {\mu}$g/min for casein. The activation energy for the alkaline protease calculated by Arrhenius equation was 3.643 kcal/mol. This enzyme hydrolyzed casein more rapidly than the hemoglobin and egg albumin.

Keywords

Streptomyces griseus HC-1141;alkaline protease