Membrane Injury of Nocardia mediterranei upon Lyophilization and Viability Depending on Rehydration Methods

동결건조법에 있어 Nocardia mediterranei의 세포막 손상과 재수화 방법에 따른 생존도

  • 이동희 (건국대학교 미생물공학과) ;
  • 이노운 (건국대학교 미생물공학과) ;
  • 최남희 (㈜종근당)
  • Published : 1992.06.01

Abstract

In order to examine the viability depending on rehydration process and membrane injury of Nocardia mediterranei upon lyophilization, We labeled $3^H$-thymidine in deoxyribonucleic acid of N. mediterrranei to obtain information on the mechanisms of injury caused by lyophilization. Suspensions of rehydrated cells were incubated with added DNase in a buffer solution. Extracellular radioactivity levels appeared to be high in the rehydrated solutions after lyophilization than freezing-thawing. Thus, the membrane systems were injured by lyophilization, but not ovenvhelmed. These considerations were confirmed by electron microscopy. In effects of rehydration, the cell membrane was seriously damaged by strong atmospheric pressure as soon as the inner ampule was opened, but this was not the case without admitting air under vacuum. N. rnediterranei cells, with no additives, were lyophilized and reconstituted without admitting air, virtually about 84% of the cells were viable.

Keywords

Lyophilization;rehydration;incorporation;membrane injury