The DNA region of rtn gene essential for resistance against N4 infection

N4에 대해 내성을 나타내는데 필요한 rtn 유전자의 부위

  • 이동환 (전북대학교 분자생물학과) ;
  • 유선미 (전북대학교 분자생물학과) ;
  • 황의욱 (전북대학교 분자생물학과) ;
  • 이영훈 (한국과학기술원 화학과) ;
  • 채건상 (전북대학교 분자생물학과)
  • Published : 1991.11.01

Abstract

N4 phage, which infects E. coli K-12 strains, could not infect E. coli K-12 strains containing rtn(resistant to N4) gene on plasmids, which was isolated from Proteus vulgaris ATCC 13315. The region of rtn gene for Rtn phenotype was reduced to the 1.7 kb HincII-AccI fragment, and rtn gene seemed to have its own promoter. This putative promoter was present in 107 bp HindII-DraI fragment, and known to be functional in E. cole K-12, which is supported by the fact that phenotype of a subclone, pRMG103A1B which does not contain the 107 bp fragment, was dependent on the existance of a functional promoter in the upstream of rtn gene, and that the 107 bp fragment had promoter activity when located in the upstream of structural gene of galactodinase of E. coli. The promoter-bearing fragment contains two overlapping putative promoter sequences, both of which show a fit in eight of twelve nucleotides with consensus sequences of E. coli promoters at the -35 and -10 regions.

Keywords

rtn gene;N4 resistance;rtn promoter;N4 phage