Secretion of Membrane-Associated Laccase in Liquid Culture of Coprinus congregatus

Coprinus congregatus의 세포막 연관 laccase의 세포외 분비

  • 김순자 (강원대학교 미생물학과) ;
  • 최형태 (강원대학교 미생물학과) ;
  • 강사욱 (서울대학교 미생물학과) ;
  • 하영칠 (서울대학교 미생물학과)
  • Published : 1991.11.01

Abstract

The hyphal tip laccase of Coprinus congregatus which is a membrane-associated enzyme and shows diffdrdnt banding patterns of PAGE analysis when compared with the enzyme of liquid culture (Choi et al. 1987) has been successfully secreted to culture medium in liquid shake culture by lowering the pH of medium to 4.0. When the fungus is cultivated in YpSs(pH 4.0) liquid, only the hyphal tip laccase is found in the medium after 6 hr incubation and there is no liquid-type enzyme when examined by PAGE analysis.

Keywords

Coprinus congregatus;enzyme secretion;liquid cultre