In vitro 계에서의 Streptozotocin 분해 및 세포내 흡수

  • 박기숙 (서울대학교 약학대학 약리학교실) ;
  • 호지숙 (서울대학교 약학대학 약리학교실) ;
  • 정진호 (서울대학교 약학대학 약리학교실)
  • Published : 1991.12.01

Abstract

Since streptozotocin has been known to be chemically stable only under acidic condition(pH 4), the spontaneous degradation and cellular uptake of streprozotocin in neutral incubation medium was determined by chemical assay. Streptozotocin levels in both phosphate buffered saline (pH 7.4) and Krebs-Henseleit buffer (pH 7.4) decreased with a half life of about 2 hours. The presence of erythrocytes or pepatocytes under the same buffers did not affect the streptozotocin degradation rate at all. However, streptozotocin levels in plasma isolated from rats decreased rapidly compared to those in neutral buffers.

References

  1. Anal. Chem. v.36 Spectrophotometric determination of streptozotocin Forist,A.A.
  2. Proc. Soc. Exp. Bio. Med. v.126 Studies on the diabetogenic action of streptozotocin Junod,A.;Lambert,A.E.
  3. Cancer Chemother. Rep. v.58 Tissue distribution of streptozotocin (NSC-85998) Bhuyan,B.K.;Kuentzel.S.L.;Graey,L.G.;Fraser,T.J.;Wallach,D.;Neil.G.L.
  4. Biochem. Soc. Trans. v.3 The metabolic fate and elimination of streptozotocin Karunanayake,E.H.;Hearse,D.J.;Mellows,G.
  5. Biochem. J. v.142 The synthesis of $[^{14}C]$ streptozotocin and its distribution and excretion in the rat Karunanayake,E.H.;Hearse,D.J.;Mellows,G.
  6. Diabetes v.35 Acetylhomocysteine-thiolactone-induced increase of superoxide dismutase counteracts the effect of subdiabetagenic doses of streptozotocin Papaccio,G.;Pisanti,F.A.;Frascatore,S.
  7. Biochem. Pharmacol. v.31 Effect of diabetes on rat liver cytochrome P-450 Past,M.R.;Cook,D.E.
  8. Biochemical Toxicology-a Practical Approach Snell,K.;Mullock,B.
  9. Cancer Treatment Reports v.66 Streptozotocin: Review of its pharmacology, efficacy, and toxicity Weiss,R.W.
  10. Diabetologia v.27 Mechanism of streptozotocin and alloxan induced damage in rat B-cells Wilson,G.L.(et al.)
  11. Nature v.294 Streptozotocin and alloxan induce DNA strand breaks and poly(ADP-ribose) synthetase in pancreatic islets Yamamoto,H.;Uchigata,Y.;Okamoto,H.