The degradation o Diazinon by hepatic monooxygenase of Pig

돼지 간중의 Monooxygenase 가 Diazinon 의 분해에 미치는 영향

  • Ryoo, Jong-Gook (Dept. of Agricultural Chemistry, Chungnam Natl. Univ.) ;
  • Lee, Kyu-Seung (Dept. of Agricultural Chemistry, Chungnam Natl. Univ.)
  • 류종국 (충남대학교 농과대학 농화학과) ;
  • 이규승 (충남대학교 농과대학 농화학과)
  • Published : 1990.12.30


Two fractions(microsomal and soluble) were prepared by ultracentrifugation(105,000G for 1hr at $4^{\circ}C$) from pig liver in order to find the major factor in Diazinon degradation. The two enzyme activities showed the same value, but Diazinon was degraded three times in microsomal fraction more than in soluble fraction. And with addition of EPN, Beam and PBO, degradation of diazinon was inhibited(29, 30 and 60%) as well as Monooxygenase activity (14, 15 and 35%) in microsomal fraction, respectively.