Change in Protein and Carbohydrate Contents in Diapausing and Non-diapausing Pupae of the Oriental Tobacco Budworm, Heliothis assulta Guenee

담배나방 휴면용과 비휴면용의 단백질과 탄수화물의 변화

  • 조국호 (서울대학교 농과대학 농생물학과) ;
  • 부경생 (서울대학교 농과대학 농생물학과)
  • Published : 1990.12.01


Studies were carried out to investigate the change in protiens and carbohydrates from diapausing pupae of the Oriental tobacco budworm, Heliothis assulta Guenee. The oxygen consumption rate of non-diapausing pupae through the whole pupal period showed an U-shaped curve, while that of diapausing pupae decreased to a mean level of 20$\mu$1/g/hr. But the rate of oxygen consumption increased in diapausing pupae before their emergence. The body weight of diapausing pupae showed almost no change during 12 days after pupation. The total contents of major carbohydrates and soluble proteins were higher in diapausing pupae than those in non-diapausing pupae and the change in carbohydrate and protein contents was more dynamic in non-diapausing pupae than that of diapausing pupae. the electrophoretic band patterns of proteins were similar in both of diapausing and non-diapausing pupae. Diapausing pupae increased their haemolmph osmolarity further when they were exposed to low temperatures.


Oriental tobacco budworm;Heliothis assulta;oxygen consumption rate;osmolarity