Microbial Control of the Tobacco Cutworm, Spodopera litura (Fab.), Using S. litura Nuclea Polyhedrosis Virus. II. Formulation of S. litura Nuclear Polyhdrosis Virus as Viral Insecticides

곤충 핵다각체병바이러스를 이용한 담배거세미나방의 생물적 방제. II. 담배거세미나방 핵다각체병바이러스의 살충제 제제화

  • Published : 1990.12.01

Abstract

Three viral insecticides were differently formulated with a nuclear polyhedrosis virus isolated from Spdodoptera litura by addition of feeding attractant, anti-precipitate of polyhedra, spreading agent, and UV-protectants. Sucrose was effective for attraction of larval feeding to increase the mortality and for protection of polyhedra from inactivation by sunlight when added 1% to 5% of sucrose solution to the formulations. Contents of additives to the formulations were 0.5% in polyvinyl alcohol to prohibit the precipitation of polyhedra and 0.1% in Triton X-100 to spread and wet the formulations to the plant. Inactivation of the virus under sunlight was decreased when added 800g of white carbon to 100 L of water in the white carbon formulation and 30% of molasses to the molasses's. In the formulation of white carbon and molasses mixtures, activation of the virus was increased when mixtured 500g of the former with 10% of the latter. Three formulations were persisted their pathogenicity more than 95% of mortality at 3 days p.i. Encapsulation of the polyhedral surface was more distinctively coated with the carbon and showed more effective in the residual effects of the white carbon than others, but the molasses more attractive for larval feeding.

Keywords

Viral insecticide;microbial control;viral inactivation;Spodoptera litura nuclear polyhedrosis virus;feeding attractant