Drect Interaction of Streptozotocin with TBA (thiobarbituric acid) in Lipid Peroxidation Analysis

Streptozotocin에 의한 lipid peroxidation 측정시 TBA법의 적합성에 관한 연구

  • 정진호 (서울대학교 약학대학 위생약학실) ;
  • 호지숙 (서울대학교 약학대학 위생약학실) ;
  • 문창규 (서울대학교 약학대학 위생약학실)
  • Published : 1990.12.01

Abstract

This study was conducted to evaluate the usage of TBA method for the analysis of lipid peroxidation induced by streptozotocin. 5 mM streptozotocin and 1 % TBA alone showed the maximum peak at 309 nm and 358 nm respectively, although no peak was observed at 532 nm which was the wavelength to determine the absorbance for TBA method. When 5 mM streptozotocin was mixed together with 1 % TBA in vitro, new peaks at 439 nm and 532 nm had been detected, suggesting TBA did interact directly with streptozotocin forming new colored products. These results suggest that TBA method is not adequate for determination of lipid lperoxidation induced by streptozotocin.