A Stable Preservation of Extracellular Nonoccluded Virions from Autographa californica Nuclear Polyhedrosis Virus Infection

Autographa californica 핵다면체 바이러스의 세포외 미봉입비리온의 안전한 보존

  • 이형환 (건국대학교 이과대학 생물학과) ;
  • 이근광 (건국대학교 이과대학 생물학과) ;
  • 이건주 (안동대학교 생물학과)
  • Published : 1990.12.01

Abstract

A stable preservation method of extracellular non-occluded virion of Autographa californica nuclear polyhedrosis virus (AcNPV) was studied. AcNPVL-1 strain infected to Spodoptera frugiperda cell line and then the culture media were centrifuged. After centrifugation the supernatant containing extracellular nonoccluded virions of the AcNPV was harvested and incubated at $4^{\circ}C$ . Even after the extracellular nonoccluded virions were incubated at $4^{\circ}C$ for about 11 years, the infectivity and multiplication property of the nonoccluded virions in the S. frugiperda cell line were normal. However the titers of the nonoccluded virions in TC-100 medium measured about 11 years ago decreased from $8.9 \times 10^7\; to \;3.8 \times 10^5$ pfu per ml. The AcNPV genome DNA fragment patterns from digestion with Hind11 and EcoRI restriction endonucleases did not change. The AcNPV nonoccluded virions were stable at $4^{\circ}C$ in the cultured medium of more than 10 years and the preservation of AcNPV nonoccluded virions at $4^{\circ}C$ is easy and useful for handling.

Keywords

Nuclear polyhedrosis virus;virus preservation