Cloning and Expression of Schwanniomyces castellii Starch Gene

Schwanniomyces castellii 전분 유전자의 Cloning과 발현

  • Park, Jong-Chun (Department of Microbiology, Chonnam National University) ;
  • Bai, Suk (Department of Microbiology, Chonnam National University) ;
  • Chun, Soon-Bai (Department of Microbiology, Chonnam National University)
  • 박종천 (전남대학교 자연과학대학 미생물학과) ;
  • 배석 (전남대학교 자연과학대학 미생물학과) ;
  • 전순배 (전남대학교 자연과학대학 미생물학과)
  • Published : 1990.12.01

Abstract

The gene encoding glucoamylase from Schwanniomyces cagtellii CBS 2863 was cloned and expressed in Saccharomyces cerevisiae. Southern blot analysis confirmed that this glucoamylase gene was derived from the genomic DNA of Schwanniomyces ccastellii and that no DNA fragments corresponding to 5.1 or 1.3 kb of Sch. casteltii DNA were detected in S. cereuisiae. The glucoamylase activity from S. cerevisiae transformant was approximately 2,000 times less than that of donor yeast. No expression was found in E. coti. The secreted glucoamylase from S. cerevisiae transformant was indistinguishable from that of Sch. eastellii on the basis of molecular weight and enzyme properties.

Keywords

Schwanniomyces;glucoamylase;gene cloning;expression