Transfer of Insecticidal Toxin Gene in Plants:Cloning of Insecticidal Protein Gene in Bacillus thuringiensis

식물세포에 살충독소 유전자의 전이: Bacillus thuringiensis 살충단백질 유전자의 클로닝

  • 이형환 (건국대학교 유전공학연구소 및 생물학과) ;
  • 황성희 (건국대학교 유전공학연구소 및 생물학과) ;
  • 박유신 (건국대학교 유전공학연구소 및 생물학과)
  • Published : 1990.12.01

Abstract

The production of delta-endotoxin crystal and the cloning of endotoxin protein gene in Bscillus thuringiensis subsp. kurstaki HD1 strain were studied. The strain produced bipyramidal crystals ($2.9\times 1.0 \mu m$) in their cells during sporulation. The B. thuringiensis contained about 10 plasmid DNA elements ranging from 2.1 to 80 kilobases. The 73 kb plasmid DNA, the 29 kb BamHI fragment and the 7.9 kb Pstl DNA fragment hybridized to the pHL probe. The 7.9 kb fragment was eluted and cloned in the PstI site of pBR322 vector and transformed into E. coli HB101, which produced insecticidal proteins killing Bornbyx mori larvae.

Keywords

Endotoxin gene cloning;B. thuringiensis toxingene