The Production and Purification of Chitinase from Aeromonas salmonicida YA7-625

Aeromonas salmonicida YA7-625에 의한 Chitinase의 생산 및 정제

  • 이강표 (연세대학교 식품공학과) ;
  • 김창남 (연세대학교 식품공학과) ;
  • 오두환 (연세대학교 식품공학과) ;
  • 유주현 (연세대학교 식품공학과)
  • Published : 1990.12.01

Abstract

A chitinase-producing bacterium, Aeromonas salmonicida YA7-625, was isolated from domestic seashore muds. The preferable medium composition for the production of chitinase was as follows: colloidal chitin 1.26% (w/v), tryptone 2.95% (w/v), $MgSO_4-7H_20$ 0.15% (w/v) and $K_2HP0_4$, 0.15% (w/v) (pH 8.5). The highest enzyme production was observed after cultivation of 48 hours at 27OC. The chitinase of Aeromonas salmonicida YA7-625 was purified successively by ammonium sulfate precipitation, affinity adsorption, hydroxylapatite column chromatography and gel filtration. The optimal temperature and pH for the activity of purified chitinase were $50^{\circ}C$ and 7.0, respectively. The molecular weight of purified chitinase was ca. 200,000 daltons and apparent Km value of it was 1.276 mglml on colloidal chitin.

Keywords

Colloidal chitin;chitinase;Aeromonas salmonicida