Produ cti on of Cyclomaltodextrin from Bacillus stearothermophilus

Bacillus stearothermophilus에 의한 Cyclomaltodextrin Glucanotransferase의 생산

  • 황진봉 (한국식품개발연구원) ;
  • 김승호 (한국식품개발연구원) ;
  • 이태경 (고려대학교 생물공학 연구소) ;
  • 양한철 (고려대학교 생물공학 연구소)
  • Published : 1990.12.01

Abstract

A microorganism capable of producing high level of extracelluar cyclomaltodextrin glucanotransferase(EC 2.4.1.19; CGTase) was isolated ’rom soil. The isolated strain No. 239 was identified as Bacillusstearothermophilus. The maximal CGTase production (about 7.0 unitslml) was observed in medium containing2% soluble starch, 0.5% defatted soybean meal, 0.1% NaH_2PO_4.2H_2O$ and 0.015% $ CaC_l2 $ with initial pH 7.0. The strain was cultured at $55^{\circ}C$ for 48 hr with reciprocal shaking. At 0.83% substrated concentration potato starch was the optimum substrate with 50.1% conversion to cyciodextrin (CD)after the reaction at $65^{\circ}C$ for 24 hr (CGTase 10 unitlg starch). Using soluble starch as substrate (5% substrate concentration, CGTase 10 unitlg starch), the maximum conversion of 40% was obtained at11 hr reaction, and the ratio of $\alpha-, \beta-$ and $\gamma$-CD production at this time were 1.0:1.3:0.4, respectively., respectively.

Keywords

Bacillus sp.;cycolmaltodextrin glucanotransferase