Transformation of Trichoderma koningii Using Isolated Nuclei

분리 핵을 이용한 Trichoderma koningii의 형질전환

  • 민경림 (태평양화화 생명공학연구소) ;
  • 박희문 (충남대학교 미생물학과) ;
  • 하영칠 (서울대학교 미생물학과) ;
  • 정재훈 (과학원 생물공학과)
  • Published : 1990.12.01

Abstract

When protoplasts from auxotrophie mutant of Trkhoderma koningii CUT121(Lys-, Met-) were mixed with isolated nuclei of wild type T. koningii ATCC 261 13 and treated with PEG solution, protrophic colonies were produced with frequency of more than 30 percent. One of segregants from prototrophic colonies showed increased xylanase activity and other polysaccharide-hydrolyzing activities comparable to those of wild type strain. Through measurement of DNA contents, induced segregation, and analysis of isozyme patterns, it was revealed that the prototrophic colonies were transformants resulted from exchange of genetic materials between the two kinds of nuclei used. These results suggest that nuclei transfer technique is more efficient than conventional protoplast fusion technique for strain improvement of Trichoderma species.

Keywords

Isolated nuclei;protoplast;transformation;Trichoderma koningii