Isolation and Identificatioh~ of a Phthalate Ester Degrading Bacterium and the Optimal Culture Conditions for Production of One Degrading Enzyme

프탈레이트 에스터 분해세균의 분리 및 분해효소의 최적 생성조건

  • Kim, Byung-O (Department of Microbiology, College of National Sciences Kyungpook National University) ;
  • Kim, Ran-Sug (Department of Microbiology, College of National Sciences Kyungpook National University) ;
  • Jin, Ing-Nyol (Department of Microbiology, College of National Sciences Kyungpook National University) ;
  • Park, Wan (Department of Microbiology, College of National Sciences Kyungpook National University)
  • 김병오 (경북대학교 자연대 미생물학과) ;
  • 김란숙 (경북대학교 자연대 미생물학과) ;
  • 진익렬 (경북대학교 자연대 미생물학과) ;
  • 박완 (경북대학교 자연대 미생물학과)
  • Published : 1990.12.01

Abstract

A strain degrading phthalate ester was isolated from a sludge of Taegu area and identified as a strain of Klebsiella. The optimum culture conditions for the protocatechuate dioxygenase production were also investigated. This strain produced the enzyme in question under the shaking cultivation at $30^{\circ}C$for the 48 hrs in the medium containing 0.1% protocatechuate as the sole carbon source, 0.1% ammonium sulfate and 0.1% yeast extract as the nitrogen source and mineral salt mixture of magnesium sulfate, sodium chloride, calcium chloride, ferric chloride, manganese sulfate, zinc sulfate and cupric sulfate. This enzyme was intracellularl j localized and probably linked to cell membrane, and induced by protocatechuate.

Keywords

Phthalate ester;protocatechuate dioxygenase;protocatechuate(PCA)