Construction of Plasmids for Overproduction of L-Phenylalanine

L-페닐알라닌 대량생산을 위한 재조합 플라스미드 구성

  • Lee, Sae-Bae (R &D Center, MiWon Co., Ltd.) ;
  • Park, Chung (R &D Center, MiWon Co., Ltd.) ;
  • Won, Chan-Hee (R &D Center, MiWon Co., Ltd.) ;
  • Choi, Duk-Ho (R &D Center, MiWon Co., Ltd.) ;
  • Lim, Bun-San (R &D Center, MiWon Co., Ltd.)
  • 이새배 ((주)미원 연구소) ;
  • 박청 ((주)미원 연구소) ;
  • 원찬희 ((주)미원 연구소) ;
  • 최덕호 ((주)미원 연구소) ;
  • 임번삼 ((주)미원 연구소)
  • Published : 1990.06.01

Abstract

For the overproduction of L-phenylalanine using Escherichia coli, the authors constructed various recombinant plasmids including pMW 10, pMW 11 and pMW 12. The $aroF{FR}$ and $pheA^{FR}$ genes for the production of L-phenylalanine were isolated from Escherichia coli MWEC 101-5 strains. The productivity and atability of Escherichia coli regulatory mutants containing recombinant plasmids were investigated to evaluate the efficiency of the $aroF^{FR}$ and $pheA^{FR}$ genes. The MWEC 101-5/pMW 11 strain produced 24.3g/l of L-phenylalanine while its stability was 73.8 percent. The specific activity of prephenate dehydratase in the MWEC 101-5/pMW 11 strain increased by 26-fold compared with that of Escherichia coli K-12.

Keywords

L-phenylalanine;recombinant plasmid;nezyme acitivity