Construction of Starch-Fermenting Yeast Using Protoplast Fusion Technique

원형질체 융합 방법을 이용한 전분 발효성 효모의 개발

  • Chun, Soon-Bai (Department of Microbiology, Chonnam National University) ;
  • Bai, Suk (Department of Microbiology, Chonnam National University) ;
  • Lee, Kee-Young (Department of Chemical Technology, Chonnam National University) ;
  • Kim, Soo-Hyun (Department of Biology, Chonnam National University) ;
  • Lee, Jin-Jong (Department of Biology, Chonnam National University) ;
  • Kim, Sang-Moon (Department of Biology, Chonnam National University)
  • 전순배 (전남대학교 미생물학과) ;
  • 배석 (전남대학교 미생물학과) ;
  • 이기영 (전남대학교 공업화학과) ;
  • 김수현 (전남대학교 생물학과) ;
  • 이진종 (전남대학교 생물학과) ;
  • 김상문 (전남대학교 생물학과)
  • Published : 1990.06.01

Abstract

Intraspecific fusion frequency between Filobasidium capsuligenum CBS6122-2 ade and met trp or met strains was $2.9\times 10^{-3}$ and $8.5\times 10^{-3}$, respectively. And intergeneric fusion frequency between Folobadidium capsuligenum CBS 4318 (cys his) and Saccharomyces cerevisiae 262-12-1 (hom3 thr1) or X2180-1A (ade thr) was $8.8\times 10^{-6}$ and $9.5\times 10^{-4}$, respectively. Nuclear fusion appears to occru in fusants between intraspecies and intergenera as strongly suggested by DNA content, nuclear staining, comparison of survival rate to UV light and mitotic segregation analysis. It was also found that $\alpha$-amylase and glucoamylase activity from intraspecific hybrids was 1.6-2.1 fold increased when compared with that from thier parents.

Keywords

Filobasidium capsuligenum Saccharomyces cerevisiae;protoplast fusion