Expression of a Yeast Superkiller Gene(SK13) in Saccharomyces cerevisiae

Saccharomyces cerevisiae에서 효모 Superkiller 유전자(SK13)의 발현

  • ;
  • Wickner, Reed B.
  • 이상기 (한국과학기술원 유전공학센터) ;
  • Published : 1990.06.01

Abstract

A yeast chromosomal superkiller gene (SK13) was cloned and expressed in $ski3^{-}$ Saccharomyces cerevisiae strains. The gene was fused to the structural region of E. coli lacZ gene at its C-terminus in a yeast-E. coli shuttle vector, pSR605. The fused gene complemented $ski3^{-}$ strains with SK13 activity and the quantitative level of expression was measured as determined by assaying $\beta$-galactosidase activity. The SDS-polyacrylamide gel electrophoresis and the Western blot analysis of this fused protein showed the immuno-reacted bands with a protein of the estimated molecular size (ca.250Kd).

Keywords

SK13;S cerevisiae;lacZ;gene fusion