Mutator effects of plasmid pKM101 and pSL4 to E. coli DNA repair

E. coli DNA 회복에 미치는 플라스미드 pKM101과 pSL4의 mutator 기능

  • 전홍기 (부산대학교 자연과학대학 미생물학과) ;
  • 이상률 (부산대학교 자연과학대학 미생물학과) ;
  • 백형석 (부산대학교 자연과학대학 미생물학과)
  • Published : 1990.06.01


The mutagenesis-enhancing plasmid pKM101 and its mutant pSL4 were introduced into Escherichia coli B/r strains possessing different DNA repair capacities ($phr^{-}, recA^{-}, uvrA^{-}, uvrB^{-}$) and determined the protection effect and mutagenecity for UV and MNNG. The mutability and protection effect of plasmid pKM101 and pSL4 were affected by different DNA repair capacity. The mutagenecity and resistance of two plasmids were increased against UV and MNNG, and plasmid pSL4 had a higher effect than pKM101. We suggest that the functional differences between pKM101 and pSL4 is due to the variety of mutator gene.


pkM101;pSL4;mutator genes;muc gene