Cloning of agrobacterium tumefaciens chromosomal virulence region

Agrobacterium tumefaciens의 염색체 DNA내에 존재하는 종양 유발 지역의 클로닝

  • ;
  • Cangelosi, G.A. ;
  • Nester, E.W.
  • 심웅섭 (고려대학교 생물학과) ;
  • ;
  • Published : 1990.06.01

Abstract

The chromosomal DNA of Agrobacterium tumefaciens contains the genes required for bacterial attachment to plant cell which is an essential atage in crown gall tumorigenesis by Ti-plasmid. In order to clone the genes, Agrobacterium tumefaciens strain A5512 was mutagenized by transposon Tn5 and two Agrobacterium tumefaciens mutants which are attachment-defective and nontumorigenic were isolated. From one of the two mutants, a chromosomal virulence region which was required for attachment to the plant cells was cloned.

Keywords

A. tumefaciens;Virulence region Cloning