A Study on the Simultaneous Analysis of Fat-Soluble Vitamins in Food Stuffs and Vitamin Products by High Performance Liquid Chromatography

고성능 액체 크로마토그래피에 의한 식품 및 비타민 제제중의 지용성 비타민의 동시 분석에 관한 연구

  • Poongzag Kim (Analytical Research and Support Section, Korea Research Institute of Chemical Technology) ;
  • Chong-Hyeak Kim (Analytical Research and Support Section, Korea Research Institute of Chemical Technology)
  • Published : 1989.02.20


The extraction method and quantitative analysis for the fat-soluble vitamins present in food stuffs and vitamin products have been investigated. The simultaneous separation and analysis of the vitamins by reverse phase high performance liquid chromatographic method was conducted using an isocratic elution with methanol : water (95 : 5) eluent on a Novapak $C_{18}$ column. The detection of vitamins was achieved by a variable wavelength UV detector. To improve the detection sensitivity detection wavelengths were set at the highest absorption bands such as 330, 265, 285, and 290nm for the respective vitamins. The analysis for the fat-soluble vitamins was finished within 40 minutes. Alkaline hydrolysis and enzymatic hydrolysis were investigated for the sample preparation; and liquid-liquid extraction and liquid-solid extraction were attempted for the extraction of vitamins. Both hydrolysis methods were turned out to be appropriate for the analysis for vitamins A, D, and E, while for the analysis of vitamin K the enzymatic hydrolysis method demonstrated better results. Diethyl ether, pentane, and n-hexane were found to give higher recovery for the liquid-liquid extraction and silica cartridge for the liquid-solid extraction.



  1. HPLC Food Anal. v.187 P. J. Van Niekerk
  2. J. Assoc. Off. Anal. Chem. v.66 M. C. Mulry(et. al.)
  3. J. Nutr. Sci. Vitaminol. v.30 T. Kobayashi(et. al.)
  4. NBS Spec. Publ. v.519 J. N. Thompson(et. al.)
  5. J. Agric. Food Chem. v.26 H. Cohen;M. Lapointe
  6. Anal. Chem. v.51 S. A. Barnett;L. W. Frick
  7. J. Assoc. Off. Anal. Chem. v.69 J. N. Thompson
  8. CRC Crit. Rev. Food Sci. Nutr. v.13 D. B. Parrish
  9. Recent Dev. Food Anal. Proc. Eur. Conf. Food Chem. v.1st A. Rougereau(et. al.)
  10. Liq. Chromatogr. Anal. Food Beverages v.2 J. W. Devries(et. al.)
  11. Analyst v.109 S.L. Reynolds;H.J. Judd
  12. J. Liq. Chromatogr. v.2 J.N. Thompson;G. Hatina
  13. J. Micronutrient Anal. v.1 H. Fukuba;T. Murota
  14. J. Liq. Chromatogr. v.4 P.A. Lotfy(et. al.)
  15. J. Assoc. Off. Anal. Chem. v.67 H. Cohen(et. al.)
  16. J. Chromatogr. Sci. v.10 R. C. Williams(et. al.)
  17. J. Chromatogr. Sci. v.16 J. W. Dolan(et. al.)
  18. J. Chromatogr. v.246 F. Zonta;B. Stancher
  19. Contemporary Practice of Chromatography C. F. Poole;S.A. Schuette