Cloning of RNA1 Gene from Saccharomyces cerevisiae

Saccharomyces cerevisiae에서 RNA1 유전자의 클로닝

  • 송영환 (부산수산대학교 미생물학과) ;
  • 고상석 (서울대학교 미생물학과) ;
  • 이영석 (서울대학교 미생물학과) ;
  • 강현삼 (서울대학교 미생물학과)
  • Published : 1989.06.01


The temperature sensitive (ts) mutation on RNA1 gene of Saccharomyces cerevisiae prevents growth at restrictive temperature ($36^{\circ}C$) by accumulation of precursor tRNA, rRNA and mRNA (Hutchison et al., 1969; Shiokawa and Pogo, 1974; Hopper et al., 1978). RNA1 gene was cloned by complementation of the temperature sensitive growth defect of an rna1-1 mutant strain and identified by retransformation and concomitant loss of recombinant plasmid on non-selective condition. By deletion mapping, it was found that RNA1 gene resides within 3.5kb of BgII fragment.