Lactobacillus sporgenes에 의한 젖산칼슘 생산 II. 젖산 칼슘 제조

The Production of Calcium Lactate by Lactobacillus sporogenes II. Production of Calcium Lactate

  • 이계근 (일동제약(주) 중앙연구소) ;
  • 김영만 (일동제약(주) 중앙연구소, 신흥전문대학 식품영양과) ;
  • 민경찬
  • 발행 : 1988.12.01


Production of calcium lactate very useful for medical supplies of Ca-therapy was obtained by lactic acid fermentation of lactobacillus sporogenes, a spore forming lactic acid bacterium. Corn steep liquor 1%, soybean enzyme hydrolysate 3%, yeast extract powder 2% can substitute for yeast extract and peptone as nutrient sort traces in fermentation medium using 10% glucose concentration. In the calcium lactate production medium containing yeast extract powder 2%, glucose 18%, CaCO3 12%, the lactic acid fermentation was carried out at 45$^{\circ}C$ for 4days with continuous agitation of 100 rpm. As results, fermentation yield was 97.5%. The five steps such as protein coagulation, decolorizing evaporating, crystallizing, and drying were carried out to harvest calcium lactate from 10l of supernatant of fermented medium to be removed cell and CaCO3. As results, 2065.0g of white crystal calcium lactate dihyrate was recovered and a yield of 84.9% was obtained.