Optimum culture conditions for production of extracellular cytosine deaminase by bacellus polymyxa YL 38-3

Bacillus polymyxa YL38-3의 세포외 cytosine deaminase 생성의 최적 배양 조건

  • 유대식 (계명대학교 자연과학대학 미생물학과) ;
  • 김대현 (계명대학교 자연과학대학 미생물학과) ;
  • 박정문 (계명대학교 자연과학대학 미생물학과) ;
  • 송형익 (경북대학교 농과대학 식품공학과) ;
  • 정기택 (경북대학교 농과대학 식품공학과)
  • Published : 1988.12.01

Abstract

The strain YL 38-3, which was capable of producing extracellular cytosine deaminase, was isolated and taxonomically examined. The isolated strain was identified to be Bacillus polymyxa YL 38-3. The optimal conditions for the enzyme production from Bacillus polymyxa YL 38-3 were investigated. The enzyme production was reached maximum level in the medium containing 0.5% glucose, 0.2% beef extract, 0.5% NaCl and 0.1% $KH_{2}PO_{4}$ (pH 6.0). And the enzyme showed the highest activity when the strain YL 38-3 was cultivated at $35^{\circ}C$ for 24 gours under the initial pH 6.0. By the additions of peptone the extracellular enzyme production was inhibited, meanwhile the intracellular enzyme production was highly stimulated. It was, therefore, deduced that peptone was related to the secretion mechanism of the enzyme from this bacterial cell.

Keywords

extracellualr cytosine deaminase;Bacllus polymyxa YL 38-3