Relationship between Sporulation and Synthesis of Alkaline Protease in Streptomyces sp.

방선균 일주에서 포자형성과 호알칼리성 단백질 분해효소의 생합성과의 관계성

  • 정병철 (서울대학교 자연과학대학 미생물학과) ;
  • 신현승 (서울대학교 자연과학대학 미생물학과) ;
  • 이계준 (서울대학교 자연과학대학 미생물학과)
  • Published : 1988.12.01

Abstract

The aims of the present studies were to understand the physiolosical and genetic characters of Streptomyces sp. isolated from soil. It revealed that Streptomyces sp. SMF301 had very fast growth rate and produced extracellular protease and heavily sporulated on rich media. It also showed $\beta$-lactamase activity and pigment production. Nonsporulating mutants were isolated after NTG or acriflavin treatment and their characters were compared with the parent strain. It was found that the mutants obtained by acriflavin treatment and ghier characters were compared with the parent strain. It was found that the mutants obtained by acriflavin treatment lost the pigment formation and $\beta$-lactamase production. Protease actibity of the mutant was lowered and the pH optimum was changed toward neutral. It was found that the changes were resulted from the reduction of alkaline protease biosynthesis in the bald mutant. Therefore it is considered that sporulation, pigment formation, $\beta$-lactamase production, and alkaline protease production in Streptomyces sp. might be controlled with a closely related relationship.

Keywords

Streptomyces;sporulation;alkaline protease