Spheroplast fusion of pseudomonas spp. using plasmid as selective marker

선별표지로 plasmid를 이용한 pseudomonas spp.의 원형질체 융합

  • 이주실 (고려대학교 이과대학 생물학과) ;
  • 이미란 (고려대학교 이과대학 생물학과) ;
  • 이영록 (고려대학교 이과대학 생물학과)
  • Published : 1988.12.01

Abstract

Antibiotic resistance plasmids (RP4, pMG1, R679 and R91-5) were used as primary selective markers to detect the fusants in Pseudomonas spp. By using plasmid marker, clones containing both plasmids of parental strains were obtained as fusants by direct selection. The spheroplast fusion was occured not only between strains of interspecies, but also between strains of intergenus such as Pseudomonas and E. coli. The frequencies of fusant formation were variable from $2.8\times 10^{-1}$ to $6.0\times 10^{-4}$ -4/. In addition, chromosomal recombinants were formed among the clones with both parental plasmids in frequencies of 0.44-1.3%. The fusant formation frequency between interspecies of intraspecies was not different markedly, but stability of plasmids in fusants correlated with the phylogenic smilarity of the parental strains.

Keywords

Pseudomonas;spheroplast fuslon;R plasmid