The change of ascorbate oxidase isozyme pattern during mycelial development of streptomyces lavendulae

Streptomyces lavendulae의 균사체 발달에 따른 ascorbate oxidase 동위효소 양상 변화

  • 이현무 (단국대학교 이공대학 미생물학과) ;
  • 김재헌 (단국대학교 이공대학 미생물학과)
  • Published : 1988.06.01

Abstract

pH decreased as the substrate mycelium developed, $\Delta$pH was 1.05-1.15, but increased after the aerial mycelium formation. The lactic acid content in culture solution showed no difference between 0.2% and 5% glucose, at which the aerial mycelium formation was repressed. The growth and development of mycelium was delayed by the lactate treatment. The activity of catalase was maximum in 24 hours after inoculation, and the wuperoxide dismutase activity showed a constant level during the developmental phases. The ascorbic acid accumulated after the aerial mycelium formation. The ascorbate oxidase isozyme of Rf 0.44 appeared, while the isozyme of Rf 0.36 desappeared during the development.

Keywords

Streptomyces lavendulae;lacteic ackd;ascorbate oxidase