Effects of Some Carbohydrates and Ammonium Sulfate on Lignin Degradation by Pseudomonas diminuta

탄수화물과 황산암모늄이 Pseudomonas diminuta의 리그닌 분해에 미치는 영향

  • 김규중 (강릉대학 생물학과) ;
  • 신광수 (서울대학교 자연과학대학 미생물학과) ;
  • 맹진수 (서울대학교 자연과학대학 미생물학과) ;
  • 성치남 (순천대학 생물학과)
  • Published : 1988.06.01

Abstract

To investigate the influence of cosubstrate supplement and ammonium sulfate on lignin degradation by Pseudomonas diminuta KM-4-2, isolated in the laboratory, the strain was cultured on the lignin media which contained lignin as a source of carbon and the culture filtrate was analyzed by Sephadex G-75 column chromatography. It was found that polymerization was not appeared unlike wood-rot fungi. When the carbohydrates were added, the peak of lignin at 280nm by UV scanning spectra of the filtrate, was significantly increased. In order to determine the effect of ammonium sulfate on the ligninolytic activity, the isolated strain was incubated in the media containing 0.1%, 0.25% and 0.5% of nitrogen concentration in the Warburg flask and the rate of oxygen uptake was esitmated by Warbuge Respirometer. As a result, the activity was maximum at 0.1% of nitrogen concentration and thereafter decreased in parallel with nitrogen concentration.

Keywords

Pseudomonas diminuta;cosubtrate;oxygen uptake;ammonium sulfate