Effect of Ginsenosides on .the Biosynthesis of Low density Lipoprotein Receptor in Cultured Chinese Hamster Ovary(CHO) Cell

인삼사포닌 (ginsenoside) 저밀도 지방단백질 수용체 생합성에 미치는 영향

  • Ju, Chung-No (Department of Biochemistry, College of Science Yonsei University) ;
  • Gang, In-Cheol (Department of Biochemistry, College of Science Yonsei University) ;
  • Lee, Hui-Bong (Department of Biochemistry, College of Natural Sciences, Kangweon National University)
  • 주충노 (연세대학교 이과대학 생화학과) ;
  • 강인철 (연세대학교 이과대학 생화학과) ;
  • 이희봉 (강원대학교 자연대학 생화학과)
  • Published : 1988.12.01

Abstract

Effect of the purified ginsenoside $-Rb_1$ and $-Rb_2$ on LDL receptor biosynthesis of CHO cell cultured in a high cholesterol medium was investigated . Cholesterol uptake by CHO cell cultured in a medium containing various amounts of cholesterol was traced and found that the cholesterol uptake was proportional to the concentration of cholesterol in the medium, and the population of LDL receptors were proportionally decreased as the increasing cholesterol level in the cell. However, when the CHO cells were cultured in the medium containing ginsenosides, no significant decrease of LDL receptor population occured. The biosynthesis of protein and RNA of the above cells was higher than that of CHO cells cultured in the absence of the ginsenosides, suggesting that the ginsenosides might stimulate LDL receptor bio-synthesis. It was also observed that the ginsenosides stimulated the biosynthesis of estradiol and progesterone from cholesterol in the CHO cell. From the above results, it seemed that the ginsenosides lowers the cholesterol level by stimulating the cholesterol metablism including steroid hormone biosynthesis, resulting in the lowering of inhibitory action of cholesterol on LDL receptor biosynthesis.

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