Associated Nitrogen Fixation in the Rhizosphere of Rice in Saline and Reclaimed Saline Paddy Soil -IV. Chemotaxis of Associative N2-fixing Bacteria to Single or Combined Sugars and Root Exudates

간척지토양(干拓地土壤)의 수도근권(水稻根圈)에서 협생질소고정(協生窒素固定)에 관(關)한 연구(硏究) -제사보(第四報). 협생질소고정균(協生窒素固定菌)의 단당(單糖) 및 복합당류(複合糖類)와 근분비물질(根分泌物質)에 대(對)한 화학주화성(化學走化性)

  • Published : 1987.12.30


A method for evaluating bacterial chemotactic responses toward several single of combined sugars and sterile mucilage from the different rice cultivars had been tested. Bacterial genus of Azospirillum, Pseudomonas and Agrobacterium were specially identified from the histosphere of different rice cultivars and graminea grasses in saline and reclaimed saline paddy soil. To evaluate chemotaxis of these strains a modification of Fendrik channel method was used. Under this condition Azospirillum lipoferum Ecc 3-1 reacted stereoisomerically fomulating the single migration ring while Agrobacterium radiobacter Ecc 1-1 and Pseudomonas sp Ecc 4-1 did not. Strains specificities of chemotaxis to the single sugar such as D(+)-glucose and D(+)-fructose were less prominent than malic and citric acid. Chemotactic responses to the combined sugar such as D-galacturonic acid and the L-aspartate were found high attracting reaction than other combined sugars. Chemotaxis of associative $N_2$-fixing bacteria to the root exudates of different rice cultivars were differed among bacterial strains and rice cultivars.